Direct PCR Reagents and Kits
Direct PCR Reagents and Kits
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Invitrogen™ Single Cell Lysis Kit
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Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.
Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.
Invitrogen™ TaqMan™ Cells-to-CT™ Express Lysis Reagents
This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit.
Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
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Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Fast or Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, Less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Frozen, Dry Ice |
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GC-Rich PCR Performance | High |
Content And Storage | • Express Lysis Solution (room temperature or 4°C) • Express ezDNase (–20°C) • SuperScript IV VILO Master Mix (–20°C) • SuperScript IV VILO No RT Control (–20°C) • TaqMan Fast Advanced Master Mix (4°C) |
Form | Liquid |
Isolation Technology | Direct cell lysis |
Kit Type | Direct Cell Lysis RT-qPCR |
Green Features | Less hazardous, less waste |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | Yes |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Target | cDNA |
Format | Kit |
Sample Type | Cultured mammalian cells |
Reaction Speed | Fast |
Label or Dye | ROX |
Purification Target | RNA |
Shelf Life | 1 Year |
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
Shipping Condition | Dry Ice |
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Content And Storage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
Reverse Transcriptase | M-MLV |
Size (Final Product) | 7 kb or less |
Final Product Type | cDNA |
Sample Type | Mammalian Cells |
Purification Time | 2 hr. |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Product Line | Ambion™, Cells-to-cDNA™ |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 2X |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | High-throughput Compatible |
Detection Method | Primer-probe |
Format | Tube |
Product Type | Fast Advanced Cells-to-Ct Kit |
Sample Type | Cell |
Reaction Speed | Fast |
Reagent Type | Cell Lysis |
Purification Target | Lysate |
Product Line | Cells-to-CT™ |
Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | M-MLV |
Green Features | Fewer resources used and less waste, Less hazardous |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | DNA Polymerase |
Format | Tube |
Product Type | Gene Expression Cells-to-C Kit |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell |
Reaction Speed | Standard |
Product Line | Ambion™, Cells-to-CT™, TaqMan™ |
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 2X |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | High-throughput Compatible |
PCR Method | 2-step RT-qPCR |
Detection Method | SYBR |
Format | Tube |
Product Type | Fast Advanced Cells-to-Ct Kit |
Sample Type | Cell |
Reaction Speed | Fast |
Reagent Type | Cell Lysis |
Purification Target | Lysate |
Product Line | Cells-to-CT™ |
Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
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Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, Power SYBR™ Green |
Detection Method | SYBR |
Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Ambion™ Fast SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
Shipping Condition | Dry Ice |
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Content And Storage | Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, Fast SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature. |
Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | M-MLV |
Purification Time | 5 min. |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 20X |
For Use With (Application) | RT-PCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Fast DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
Sample Type | Cells |
Reaction Speed | Fast |
Label or Dye | SYBR Green |
Hot Start | Built-In Hot Start |
Product Line | Cells-to-CT™, SYBR™ |
Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.
Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The TaqMan™ Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.
Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
Shipping Condition | Dry Ice |
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Purity or Quality Grade | LD (Low DNA) |
Reverse Transcriptase | M-MLV |
Purification Time | 5 min. |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 20X |
For Use With (Application) | RT-PCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cells |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Hot Start | Built-In Hot Start |
Product Line | Cells-to-CT™, SYBR™ |